Uaeb Workshop Series I Proteomics Workshop

AY2015/2016 Semester 2

The aim of this workshop is to train students to apply proteomic strategy to biological and biomedical research. Students will use tissue and blood samples from large animal (pig and/or dog) models of ischemia heart disease to discover molecular biomarker and disease mechanism by analyzing the samples using mass spectromertry-based proteomic approach. Students won?t handle or do operation on the animal. The tissue and blood samples will be provided by a cardiac research laboratory. At the start of each day, a seminar will be presented by the faculty in-charge to allow students to understand the experiment and to learn the principles of various proteomic methods to be used. Then, the faculty in-charge and teaching assistants will teach the students how to prepare and analyze the proteomic samples, and how to apply bioinformatic tools for data analysis and interpretation. In this practical workshop, students will learn protein extraction and purification using mass spectrometry compatible buffers, shotgun proteomic sample preparation, HPLC protein separation and fractionation, hand-on mass spectrometry skills, mass spectrometry data interpretation, protein sequence database search for protein identification and post-translational modifications characterization. Students will also learn data mining to discover biomarker candidates and underlying molecular disease mechanism. At the end of every day, students will need to collate and discuss their data with the faculty in-charge. This will be conducted in the SMART classrooms. At the end of the course, students will need to submit a report and make presentations that will be graded by the faculty in-charge. As there is a limitation of the mass spectrometry instrument time for this course, the maximum class size will be constrained to 15 students.